Toxicogenomics (TGx) has contributed significantly to toxicology and now hasgreat potential to support moves towards animal-free approaches in regulatorydecision making. Here, we discuss in vitro TGx systems and their potentialimpact on risk assessment. We raise awareness of the rapid advancement ofgenomics technologies, which generates novel genomics features essential forenhanced risk assessment. We specifically emphasize the importance of repro-ducibility in utilizing TGx in the regulatory setting…